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KMID : 0361720050160040300
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Korean Journal of perinatology
2005 Volume.16 No. 4 p.300 ~ p.308
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Search for Differentially Expressed Genes in Preterm Human Placentae and Their Membranes by Annealing Control Primer System
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Ko Hyun-Sun
Shin Jong-Chul
Park Tae-Chul
Kil Ki-Cheol
Kim Soo-Pyung
Kim Yeon-Hee
Chung Dae-Young
Yang Dong-Eun
Jeong Ju-Hee
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Abstract
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Objective: The aim of this study was to investigate the gene expression profiles using GeneFishingTM
kit in human placentae and their membranes delivered at preterm caused by preterm labor.
Methods: Specimens were obtained from placenta, chorion, and amnion delivered at preterm and term, respectively. Total RNAs were isolated from each specimen. Thereafter, the profiles of expression genes between preterm and term specimens were compared using a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) that involves annealing control primers (ACPs) to identify the genes expressed differentially and screened by basic local alignment search tool (BLAST) search.
Results: Using 20 ACPs, 13 differentially expressed genes (DEGs) were identified and sequenced. 7 of them were expressed up-regulation, while 6 were expressed down-regulation in preterm deliveries. A BLAST searches revealed that 11 were known genes and 2 were unknown genes. Among known genes, up-regulated genes were insulin-like growth factor II associated protein, vigilin, acyl-Coenzyme A dehydrogenase, tissue inhibitor of metalloproteinase 1 (TIMP1), ribosomal protein S26 (RPS26), follistatin-like 1 (FSTL1) and down-regulated genes were two mitochondrial DNAs, ribosomal protein S28 (RPS28), transglutaminase 2 (TGM2), heparin sulfate proteoglycan (HSPG, perlecan).
Conclusion: This study shows that the ACP system is a good method for the identification of preterm-related genes. Furthermore, this study suggests that further analysis of the differentially expressed genes in preterm we have identified should provide insights into the molecular basis of preterm delivery caused by preterm labor.
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KEYWORD
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Preterm, GeneFishing, Annealing control primers, ACPs
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